Development and Application of Triple Specific Proximity Ligation Assays (3PLA)
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منابع مشابه
Development and Application of Proximity Assays for Proteome Analysis in Medicine
de Oliveira, F. M. S. 2018. Development and Application of Proximity Assays for Proteome Analysis in Medicine. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1400. 60 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0164-8. Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodi...
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Efficient and precise detection techniques, along with extensive repertoires of specific binding reagents, will be needed to meet the challenges of proteome analyses. The recently established proximity ligation mechanism enables sensitive high-capacity protein measurements by converting the detection of specific proteins to the analysis of DNA sequences. Proximity probes containing oligonucleot...
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The emergence of targeted therapies for cancer has created a need for the development of companion diagnostic tests. Assays developed in recent years are aimed at determining both the effectiveness and safety of specific drugs for a defined group of patients, thus, enabling the more efficient design of clinical trials and also supporting physicians when making treatment-related decisions. Immun...
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BACKGROUND Sensitive methods are needed for biomarker discovery and validation. We tested one promising technology, multiplex proximity ligation assay (PLA), in a pilot study profiling plasma biomarkers in pancreatic and ovarian cancer. METHODS We used 4 panels of 6- and 7-plex PLAs to detect biomarkers, with each assay consuming 1 microL plasma and using either matched monoclonal antibody pa...
متن کاملSelf-assembly of proximity probes for flexible and modular proximity ligation assays.
Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which antibody-based detection of a target protein via a DNA ligation reaction of oligonucleotides linked to the antibodies results in the formation of an amplifiable DNA strand suitable for analysis. Here we describe a faster and more cost-effective strategy to construct the antibody-based proximity ligatio...
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